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pentr d topo vector backbone  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pentr d topo vector backbone
    Pentr D Topo Vector Backbone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+d+topo+vector+backbone/bio_rxiv__2023__09__20__558714-188-23-26
    Average 86 stars, based on 1 article reviews
    pentr d topo vector backbone - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: A paradigm for regulation at the effector interface with RNA-binding proteins
    Article Snippet: .. Plasmids for transient expression of Gal4-tagged UNK mutants ( and ) were created using the Gateway cloning strategy where UNK mutants in the pENTR/D-TOPO vector backbone (Thermo Fisher Scientific, K240020) were transferred in an LR reaction using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific, 11791020) into the pDEST-pcDNA3-Gal4 vector (generated by insertion of the ccdB cassette into the pcDNA3-Gal4 construct ; gift from Fei Lan) following manufacturer’s protocol. .. The entry clones were generated by first inserting the full-length mouse UNK WT that was amplified by PCR from the pTtight-UNK-IGPP vector using the Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, 11789020) into the pENTR/D-TOPO vector, yielding pENTR-UNK.

    Cloning:

    Article Title: A paradigm for regulation at the effector interface with RNA-binding proteins
    Article Snippet: .. Plasmids for transient expression of Gal4-tagged UNK mutants ( and ) were created using the Gateway cloning strategy where UNK mutants in the pENTR/D-TOPO vector backbone (Thermo Fisher Scientific, K240020) were transferred in an LR reaction using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific, 11791020) into the pDEST-pcDNA3-Gal4 vector (generated by insertion of the ccdB cassette into the pcDNA3-Gal4 construct ; gift from Fei Lan) following manufacturer’s protocol. .. The entry clones were generated by first inserting the full-length mouse UNK WT that was amplified by PCR from the pTtight-UNK-IGPP vector using the Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, 11789020) into the pENTR/D-TOPO vector, yielding pENTR-UNK.

    Plasmid Preparation:

    Article Title: A paradigm for regulation at the effector interface with RNA-binding proteins
    Article Snippet: .. Plasmids for transient expression of Gal4-tagged UNK mutants ( and ) were created using the Gateway cloning strategy where UNK mutants in the pENTR/D-TOPO vector backbone (Thermo Fisher Scientific, K240020) were transferred in an LR reaction using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific, 11791020) into the pDEST-pcDNA3-Gal4 vector (generated by insertion of the ccdB cassette into the pcDNA3-Gal4 construct ; gift from Fei Lan) following manufacturer’s protocol. .. The entry clones were generated by first inserting the full-length mouse UNK WT that was amplified by PCR from the pTtight-UNK-IGPP vector using the Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, 11789020) into the pENTR/D-TOPO vector, yielding pENTR-UNK.

    Generated:

    Article Title: A paradigm for regulation at the effector interface with RNA-binding proteins
    Article Snippet: .. Plasmids for transient expression of Gal4-tagged UNK mutants ( and ) were created using the Gateway cloning strategy where UNK mutants in the pENTR/D-TOPO vector backbone (Thermo Fisher Scientific, K240020) were transferred in an LR reaction using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific, 11791020) into the pDEST-pcDNA3-Gal4 vector (generated by insertion of the ccdB cassette into the pcDNA3-Gal4 construct ; gift from Fei Lan) following manufacturer’s protocol. .. The entry clones were generated by first inserting the full-length mouse UNK WT that was amplified by PCR from the pTtight-UNK-IGPP vector using the Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, 11789020) into the pENTR/D-TOPO vector, yielding pENTR-UNK.

    Construct:

    Article Title: A paradigm for regulation at the effector interface with RNA-binding proteins
    Article Snippet: .. Plasmids for transient expression of Gal4-tagged UNK mutants ( and ) were created using the Gateway cloning strategy where UNK mutants in the pENTR/D-TOPO vector backbone (Thermo Fisher Scientific, K240020) were transferred in an LR reaction using the Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific, 11791020) into the pDEST-pcDNA3-Gal4 vector (generated by insertion of the ccdB cassette into the pcDNA3-Gal4 construct ; gift from Fei Lan) following manufacturer’s protocol. .. The entry clones were generated by first inserting the full-length mouse UNK WT that was amplified by PCR from the pTtight-UNK-IGPP vector using the Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, 11789020) into the pENTR/D-TOPO vector, yielding pENTR-UNK.



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    pentr d topo vector backbone  (Thermo Fisher)
    86
    Thermo Fisher pentr d topo vector backbone
    Pentr D Topo Vector Backbone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+d+topo+vector+backbone/bio_rxiv__2023__09__20__558714-188-23-26
    Average 86 stars, based on 1 article reviews
    pentr d topo vector backbone - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

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